Latest technologies from King's College London

NQR Landmine Clearance

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 02:03:00 GMT

ARIES – Advanced Radiofrequency-spectroscopic Intelligent Explosives Sensor Mine, not Mine-like: Landmines and other explosives remnants of conflict cause thousands of casualties and keep millions of acres of land out-of-use long after conflicts end. Current approaches detect anomalies – mine-like objects or metal in the ground – or use animals (dogs, rats, bees) to “sniff” for traces of explosives vapour, but it has long been recognized that there is a need in some scenarios (cluttered terrain, ground contaminated with trace explosives from detonations...

Bio-compatible Random Lasers for Biosensing Applications

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 02:01:57 GMT

Researchers at King’s College London have achieved substantial innovation in the field of biosensing with the concept of sensing by lasing. The researchers at King’s College London have constructed a bio-compatible random laser made out of materials such as silk with a disordered nanostructure that can be used as a novel generation biosensor. This concept of sensing by lasing is a novel innovation in the field of biosensing. It will have applications in areas such as in vivo sensing of biological activity. In a random system, the disordered matrix folds the optical paths insid...

Direct 3D printing of two-component silicone

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 02:00:25 GMT

The Problem Facial/body prostheses are often required for patients who have lost facial/body parts, such as an ear, nose, eye, finger, hand or breast. These defects can result from trauma, congenital malformations or diseases such as cancer. Improvements in medicine, surgical techniques and in particular cancer survival rates are resulting in increasing numbers of patients who require prostheses. Typically, facial/body prostheses are manufactured by a lengthy multi-step process that involves taking an impression from the patient, hand carving the missing defect, creating a two or th...

Reducing Artifacts in Gradient-Echo MRI using Novel Radiofrequency Pulses

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 02:00:21 GMT

ProblemGradient-echo magnetic resonance imaging (MRI) is used extensively to reveal the structure, and measure the function, of different areas of the body. One particularly exciting application is functional MRI, which can be used to produce maps of brain activity in a subject performing a mental task. Unfortunately signal dropout, caused by differences in the magnetic susceptibilities of materials in the human body, adversely affects gradient-echo images. As a consequence there are areas of the image where the structure and function cannot be determined. This is a par...

All-Optical Signal Modulation

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 02:00:10 GMT

Summary The large bandwidth obtained using optical signals has led to the transmission and reception of vast amounts of information. Recent technologies have achieved a 1Tbps bandwidth thanks to photonic integrated circuits (PIC). This bandwidth is being further increased, as required by several new applications (Business analytics, Telemedicine, Content sharing, etc). Current PICs in the market can only multiplex, transmit, receive and demultiplex optical signals. Researchers from King's College London have invented a novel optical modulation technology based on the high and ultrafast n...

Inexpensive, Ultrasensitive Optical Hydrogen Sensor

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 01:59:40 GMT

Hydrogen detected by the naked eye! Researchers at King’s College London have developed an inexpensive, robust and ultrasensitive hydrogen detector based on a new type of optical metamaterial with sensitivity so large, 2% Hydrogen can even be detected by the naked eye! Creating a safe environment Hydrogen is increasingly used in a host of industrial applications, particularly in the petrochemical and chemical industries and as engineering challenges are overcome and costs reduced, it is increasingly being used as a fuel in conventional vehicular transportation. Hydrogen ...

Pentraxin 3 (PTX3) oxidation state as Prognosis Marker for Sepsis

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 01:58:06 GMT

Technology Overview: A global, unbiased, cardiac tissue-based proteomics approach for biomarker discovery in a mouse model of sepsis identified pentraxin 3 (PTX-3) as one of the most differentially expressed proteins in cardiac tissue from septic mice. PTX-3 accumulated as an octamer due to disulphide-bond formation, most notably in heart, aorta, kidney and lung. Octameric PTX-3 was detectable in mouse serum. A proof-of-principle clinical study was conducted with serum samples from 31 sepsis patients admitted to the ICU. On admission to the ICU, there was no difference in octameric, ...

Predictive Marker for Pre-Term Birth

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 01:54:16 GMT

A small antimicrobial peptide (elafin) predicts risk of developing a short cervix and spontaneous pre-term birth (sPTB) relatively early pregnancy (~ 12-14 weeks of pregnancy)Elevated elafin concentrations in cervico-vaginal fluid (CVF) ) predict cervical shortening from 14 weeks’ and remain high when cervical shortening is first detected. Raised elafin concentrations <24 weeks’ are associated with SPTB <37 weeksTechnology OverviewPreterm birth (<37 weeks’ gestation) is responsible for over 15 million births and a million neonatal deaths annually. A growing bod...

FODMAP App for people with Irritable Bowel Syndrome

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 01:50:04 GMT

BackgroundFoodMaestro has collaborated with King’s College London and Guy’s and St Thomas’ NHS Foundation Trust to create a new app to help people with IBS whose Registered Dietitian has recommended that they follow a low FODMAP* diet: http://www.foodmaestro.me/fodmap-app/. A key feature of the new app is the ability to quickly and easily find suitable foods to eat from over 30,000 ingredients and 100,000 products in leading UK supermarkets. The FODMAP by FM app is free to download on IOS and android for 14 days, with a £3.99 annual subscription thereafter. T...

Cardiac Myosin Binding Protein C (MyC): Diagnostic Marker of Cardiac Injury

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 01:44:12 GMT

Technology Overview: Elevated levels of Cardiac troponins (cTn) reflect the occurrence of cardiac muscle necrosis and have emerged as the gold standard biomarker and are incorporated into the universal definition of acute myocardial infarction (AMI) Prof Marber's group has identified a cardiac specific biomarker, cardiac myosin-binding protein C (MyC) that may offer benefits over the traditional troponin measurements. Early clinical data has demonstrated that levels of cardiac myosin-binding protein C (MyC), accumulate and disappear more rapidly than troponin following the onset...

Optical Switch

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 01:41:01 GMT

Summary One of the current aims of nanotechnology is the replacement of ‘slow’ electronic devices with ‘fast’ photonic (light based) ones. Vertical-Cavity Surface-Emitting Lasers (VCSELs) are a promising technology for optical devices as they are compact, inexpensive and power efficient. VCSELs have the second highest production volume of all semiconductor lasers owing to the large number of commercial applications such as Photonic Integrated Circuits (PICs), data transmission, printing and computer mice. In this context, Researchers from King's College London have inve...

Counterfeit Drug Detection

inteum.test@kcl.ac.uk — Tue, 22 Nov 2016 01:40:56 GMT

Non-invasive, non-destructive authentication of packaged medicines Safe Medicines, Good Health: In 2013, the United Nations Human Rights Council (UNHCR) adopted a resolution (A/HRC/RES12/24) on access to medicines that stressed “the responsibility of States to ensure access to all, without discrimination, of medicines, in particular essential medicines, that are affordable, safe, effective and of good quality”. Current methods for authenticating medicines rely on optical spectroscopy or wet chemistry and for best effect require that the pill be removed from the packaging...

Non-Ionising Imaging

inteum.test@kcl.ac.uk — Tue, 05 Aug 2014 05:48:06 GMT

Technology Overview The technology uses light at a given near infrared wavelength [NIR] to create a transmission image of dental caries (decay). Healthy hard tissues (enamel, dentine and cementum) tend to ‘guide’ light of this wavelength, leading to a bright image, whereas decay and other inhomogeneities cause optical scattering. This produces high definition images of dental caries. This is very well illustrated in the lower image below, where the caries is obviously visible. The device is designed to utilize commercial-off-the-shelf technology for production at commercial sc...

Method to identify bioburden in a root canal following preparation

inteum.test@kcl.ac.uk — Tue, 05 Aug 2014 05:45:33 GMT

The ProblemBacteria remaining in the root canal (RC) space at the time of completion of a root canal treatment (RCT) can lead to a persistent or secondary infection, which requires revision of the treatment. Currently, there are no methods in widespread use to detect bacterial presence within the root canal space in a fast and reliable manner. Such methods are especially relevant in determining the endpoint in root canal treatments to improve the success rates and the longevity of the treatment in preserving the tooth. The SolutionA new optically-based spectroscopic technique has be...

Imaging of Unstable Atherosclerotic Plaque via a Novel Magnetic Resonance (MR) Contrast Agent

inteum.test@kcl.ac.uk — Tue, 05 Aug 2014 05:44:21 GMT

The ProblemHeart attack (acute myocardial infarction) is predominantly caused by plaque in blood vessels rupturing or eroding. Biological processes found to be associated with plaque progression and destabilization are inflammation, outward vessel wall remodelling and dysfunctional matrix synthesis and degradation. The majority of plaques causing a heart attack do not cause a significant stenosis and are defined by their composition and biological activity. Thus, detection of vulnerable plaques with X-ray or computed tomography (CT) has shown little success. The Solution...

Saliva Guard (Xerostomia Device)

inteum.test@kcl.ac.uk — Tue, 05 Aug 2014 05:41:26 GMT

ProblemDebilitating oral dryness (Xeorstomia) is a common, poorly treated component of orofacial cancer radio/chemotherapy and of Sjogren’s disease (an age-related onset auto-immune condition). A device is needed to enhance patient quality of life by reducing pain, ameliorating swallowing difficulty and rampant decay while improving speech and sleep quality by effective oral delivery of artificial saliva and associated therapeutics. SolutionThe research team have developed a simple device, similar to an irrigated gum-shield, to prevent the mouth from d...

Segment Based MR-Compatible catheter steering mechanism

inteum.test@kcl.ac.uk — Fri, 18 Jul 2014 07:32:45 GMT

The ProblemIncreasingly clinicians are using cardiac ablation to cure atrial fibrillation. During cardiac ablation, energy is delivered through a catheter to areas of the heart muscle causing the abnormal heart rhythm. This energy “disconnects” the pathway of the abnormal rhythm. Recently, the procedures have been extended to more complex arrhythmias, such as scar-related ventricular tachycardia, but with more modest clinical success. This is largely related to the technical difficulties of electrical mapping and ablation by manipulating catheters within the...

Composites for Bone Replacement and Bone Scaffolds

inteum.test@kcl.ac.uk — Fri, 18 Jul 2014 07:32:43 GMT

The ProblemA large proportion of orthopaedic surgical procedures require bone grafts and with the growing demands there still remains a need to design synthetic bone graft that mimics the structure and composition of bone with good surgical handling properties. The SolutionThe project team has developed a completely synthetic composite which incorporates both bioactive ceramic and polymer components in desired ratios to form a mechanically tough material which actively encourages biomineralisation and cell attachment in body fluid. The ceramic component is a new class of calcium ph...

Candida: Anti-fungal target and immunotherapeutic candidate

inteum.test@kcl.ac.uk — Thu, 03 Jul 2014 05:22:50 GMT

The Problem Candida albicans is an opportunistic fungal pathogen, which normally exists as a commensal of the oral cavity and gastrointestinal tracts and frequently causes of superficial vaginitis infections. Moreover, common clinical procedures, such as gastrointestinal surgery, implantation of a central venous catheter or antibiotic treatment are major risk factors for life-threatening systemic candidiasis. Systemic candidiasis is now the third most common cause of h...

Assay for determining glycosyltransferase activity

inteum.test@kcl.ac.uk — Thu, 03 Jul 2014 05:22:38 GMT

SummaryThe glycosyltransferase (GT) activity assay provides a fluorescent-based, high-throughput compatible format for investigating the enzyme substrate binding of glycosyltransferases that use uridine diphosphate (UDP) sugars as donor substrates. The key component of the assay is a novel type of fluorescent UDP-sugar derivative which facilitates dual utility of the assay; it can be used in i) a binding assay, e.g. for the identification of GT inhibitors (ligand displacement assay) or ii) the investigation of the sugar-nucleotide specificity of putative GTs and other sugar-nucleotide-dependen...

HIV-1 integrase (IN) monoclonal antibody - Clone IN-2

inteum.test@kcl.ac.uk — Thu, 08 May 2014 01:00:17 GMT

Reagent nameHIV-1 integrase (IN) monoclonal antibody (#IN-2) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 integrase protein (clade B, HXB-3 isolate) RelevanceHIV-1 expresses the integrase (IN) protein as part of the polymerase gene. It is the viral enzyme that catalyses the insertion of viral DNA into the chromosomal DNA of an infected cells. Integrase is essential for viral growth and is a therapeutic target. Cross-reactivityNo cross-reactivity with no...

Mouse anti-human actin antibody - Clone MM2/193

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:59:46 GMT

Reagent NameMouse anti-human actin antibody ImmunogenHuman platelet membranes. RelevanceActin is a major protein found in most cells and is important in cell motility and contraction. It is a major component of the cytoskeleton. Cross reactivityRecognises human actin as a 42-47 kDa protein in platelet lysates. SpecificityEpitope is not mapped. Tested ApplicationsWestern blotting. Raised inMouse ClonalityMonoclonal

Human-APOBEC3G Polyclonal Antibody

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:59:44 GMT

APOBEC3G is a human protein with natural antiviral activity, especially against HIV. As such, it is currently the subject of intense scientific investigation, and may lead to the development of new therapeutics useful for treating HIV/AIDS. Indirect Immunofluorescence HeLa cell monolayers were transfected with an APOBEC3G expression vector, fixed after 24 hr and hybridised with the anti-APOBEC3G serum (1:500 dilution). Bound antibody was detected using an Alexa Fluor 594-conjugated goat secondary antibody and imaged by confocal microscopy. APOBEC3G is seen (in green) di...

Mouse anti- human filamin antibody - Clone PM6/317

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:59:35 GMT

Reagent NameMouse anti- human filamin antibody ImmunogenHuman platelet membranes RelevanceFilamin (also called actin binding protein) is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site. It is present in smooth muscle, fibroblasts, platelets and lymphocytes. Cross reactivityChicken, Guinea Pig , Human, Rat, Rabbit SpecificityRecognizes full length Human filamin (actin-binding protein; 270-280 kDa) as well as the 190 kDa N-terminal ca...

HIV-1 p24 Gag monoclonal antibody - Clone 24-4

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:59:25 GMT

Reagent NameHIV-1 p24 Gag monoclonal antibody (#24-4) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 Gag protein (clade B, HXB-3 isolate) Relevancep24 is encoded by the HIV gag gene and is an important structural component of the capsid core of the HIV particle Cross reactivityCross-reactivity with a ~65 kDa species in western blot SpecificityEpitope not yet mapped Tested applicationsWestern blots for clade B isolates of HIV; immunofluorescence/FACS ass...

HIV-1 p24 Gag monoclonal antibody - Clone 24-3

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:59:18 GMT

Reagent NameHIV-1 p24 Gag monoclonal antibody (#24-3) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 Gag protein (clade B, HXB-3 isolate) Relevancep24 is encoded by the HIV gag gene and is an important structural component of the capsid core of the HIV particle Cross reactivityNot tested SpecificityEpitope not mapped Tested applicationsWestern blots for clade B isolates of HIV; immunofluorescence assays. (not useful for immunoprecipitation assays)...

Mouse anti-human Integrin beta 3 (CD61) antibody - Clone PM6/13

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:59:14 GMT

Reagent NameHuman Integrin beta 3 antibody ImmunogenHuman platelet membranes RelevanceIntegrin beta 3 is also known as glycoprotein IIIa and in a complex with the integrin alpha 2b subunit forms the receptor for fibrinogen, fibronectin and von Willebrand Factor on activated platelets. Integrin beta 3 can also complex with alpha V integrin. The antibody inhibits platelet aggregation, inhibits the binding of soluble fibrinogen to activated platelets and also inhibits adhesion of activated platelets to immobilised fibrinogen, fibronectin or vWF. Cross reactivityHuman and bab...

Mouse anti-human Vinculin antibody - Clone V284

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:58:24 GMT

Reagent NameMouse anti-human Vinculin antibody ImmunogenPurified human platelet vinculin RelevanceVinculin is a 117 kDa membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. Cross reactivityHuman, rabbit, rat, chicken, mouse. SpecificityEpitope not mapped. Recognises vinculin and a 130 kDa protein in platelets and other cells. Tested ApplicationsWestern blotting, immunoprecipitation, immunohistology Raised inMouse ClonalityMonoclonal IsotypeIgG1 ReferencesPorter ...

Optical endoscope for vascular imaging and concurrent oximetry instrument for non surgical tissue vascular assessment.

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:57:50 GMT

TechnologyA clinic based optical instrument that allows a doctor to examine the blood supply to a particular tissue, without need for surgical biopsy. Based on multiple wavelength analysis of light reflected by sub-cutaneous fatty layers, this instrument will allow high-resolution video-rate mapping of capillary blood flow and oxygen carriage from the same tissue simultaneously. This is not clinically possible with any instrument at present. Market Vascular imaging has applications in surgical, medical and research disciplines, such as transplant surgery and assessment of potentiall...

Novel, cadmium-free, fluorescent and magnetic nanoparticles suitable for surface functionalisation.

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:57:49 GMT

Summary King’s Inventors have developed a robust method for the synthesis of water soluble, multicoloured fluorescent-magnetic nanoparticles which do not involve the use of quantum dots, with their associated potential toxicity issues. These lipid encapsulated nanoparticles are made from well characterised, widely available conjugated polymers (also known as light emitting polymers) and may include a magnetic component. If Iron Oxide is incorporated the nanoparticles respond to an external magnetic field. The particles maintain strong fluorescence ...

Mouse anti-human integrin alpha 2b (CD41) antibody - Clone PM6/248

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:57:39 GMT

Reagent NameMouse anti-human integrin alpha 2b (CD41) antibody ImmunogenHuman platelet membranes RelevanceIntegrin alpha 2b is also known as glycoprotein IIb and in a complex with the integrin beta 3 subunit forms the receptor for fibrinogen, fibronectin and von Willebrand Factor on activated platelets. The antibody activates platelets under stirring conditions and inhibits at higher doses . The antibody inhibits binding of fibrinogen, fibronectin and vWF in static adhesion assays and inhibits binding of soluble fibrinogen and fibronectin to ADP activated platelets. Cross reacti...

Human Thyrotrophin Receptor Monoclonal Antibody - Clone 2C11

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:57:35 GMT

The human thyrotrophin receptor (TSHR) is found primarily in epithelial cells of thyroid follicles where it induces (by cAMP) the phospholipase C and protein kinase A signal transduction pathways. TSHR is the major pathogenic autoantigen in human autoimmune thyroid Graves’ disease. LanesTSHRGlycosylated TSHRTSHR - GSTResidues 125-369 TSHR - GST Product TypeHybridoma for monoclonal antibodyImmunogenBacterially expressed extracellular domain (residues 1-415 of human TSHR fused to GST)Cross ReactivityCross-reacts with human and theoretically with rat, pig, sheep,...

HIV-1 p17 Gag monoclonal antibody - Clone 17-1

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:56:19 GMT

Reagent NameHIV-1 p17 Gag monoclonal antibody (#17-1) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1. ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 p17 Gag protein (clade B, HXB-3 isolate) Relevancep17 is encoded by the HIV gag gene and is an important structural component of the HIV particle Cross reactivityNot tested SpecificityEpitope not mapped Tested applicationsWestern blots for clade B isolates of HIV; immunoprecipitation assays; immunofluorescence/FACS assays Raised in Mouse Clonality...

Mouse Anti-T Cell / Neutrophil Marker Antibody - Clone RPN3/57

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:56:16 GMT

Reagent NameMouse Anti-T Cell / Neutrophil Marker Antibody ImmunogenRabbit peritoneal neutrophils RelevanceUseful for the detection of rabbit neutrophils and T cells Cross reactivityNot tested SpecificityRecognises an antigen expressed strongly on neutrophils, thymocytes, a subset of T cells and platelets. Epitope not identified. Tested ApplicationsFlow cytometry and immunohistochemistry Raised inMouse ClonalityMonoclonal IsotypeIgG1 ReferencesWilkinson JM et al. (1992) Immunol. 76: 625-30. Wilkinson JM et al. (1993) J Pathol. 170: 315-320

Mouse anti-human SERCA 3 antibody - Clone PL/IM430

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:56:11 GMT

Reagent NameMouse anti-human SERCA 3 antibody ImmunogenHuman platelet intracellular membranes RelevanceSERCA 3 is the sarco-endoplasmic reticulum Ca2+ ATPase that sequesters Ca2+ into intracellular stores of mammalian cells. This antibody inhibits Ca2+ sequestration and Ca2+ATPase activity mediated by human SERCA3. Cross reactivityHuman SpecificityEpitope has recently been described to be between amino acids 1-236 of human SERCA3. PL/IM430 recognises a 100kDa SERCA3 protein in platelet lysates. Tested ApplicationsWestern blotting, immunoprecipitation, immunohistochem...

HIV-1 Rev monoclonal antibody - Clone Rev-4

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:56:07 GMT

Reagent NameHIV-1 Rev monoclonal antibody (#Rev-4) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 Rev protein (clade B, HXB-3 isolate) RelevanceHIV-1 expresses the trans-activator protein Rev, which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. Cross-reactivityNo cross-reactivity with non-HIV-1 Rev proteins has been observed&nbs...

A method for reducing or eliminating non resonant background obtained during CARS spectroscopy

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:55:35 GMT

CARS Microscope - Label Free Imaging: The Best of Both Worlds - Researchers at King’s College London have developed a simple and robust optical way of acquiring spontaneous Raman spectra with the speed and sensitivity of CARS. The instrument gives linear, quantitative, NRB-free signals for any laser system capable of generating CARS, including both narrowband and broadband systems. Broadband micro-Raman spectroscopy using supercontinuum laser sources can therefore give spectra equivalent to conventional linear Raman spectroscopy, but with significantly enhanced signal le...

Human Complement C1q Monoclonal Antibody - Clone IA4

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:55:05 GMT

Research tool for:ImmunohistochemistryIsolation of C1q complexes C1q is a sub-component of the first complement component C1. C1q binds to IgG and IgM immune complexes, this activates C1r and C1s, which are also bound to C1q and so initiates the classical pathway of complement activation. C1q deficiencies are observed in agammaglobulinemia, acute glomerular nephritis, systemic lupus erythematosus, and chronic glomerular nephritis. Product TypeHybridoma for monoclonal antibodyImmunogenPurified human C1qCross ReactivityNo known cross reactivity with other tissue antigensSpe...

HIV-1 Vif Monoclonal Antibody - Clone 319

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:54:41 GMT

Reagent NameHIV-1 Vif monoclonal antibody (#319) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 Vif protein (clade B, HXB-3 isolate) RelevanceHIV-1 expresses the viral infectivity factor (vif) which induces productive viral replication by inactivating the host cell anti-HIV factor APOBEC3G Cross-reactivityNo cross-reactivity with non-HIV-1 Vif proteins has been observed SpecificityEpitope not yet mapped Tested applicationsWestern blots; immunofluorescence a...

Human-APOBEC3F Polyclonal Antibody

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:54:40 GMT

APOBEC3F is a human protein with natural antiviral activity, especially against HIV. As such, it is currently the subject of intense scientific investigation, and may lead to the development of new therapeutics useful for treating HIV/AIDS. Expression and virion incorporation of APOBEC3F proteinsImmunoblot analysis of APOBEC3F wild-type (WT) and mutant proteins was performed on both 293T cells transfected with APOBEC3F expression plasmids and concentrated virus produced from these cells. Equivalent amounts of protein were loaded, as judged by amounts of the cellular protein 14-3-3 (...

HIV-1 p24 Gag monoclonal antibody - Clone 24-2

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:54:29 GMT

Reagent NameHIV-1 p24 Gag monoclonal antibody (#24-2) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 p24 Gag protein (clade B, HXB-3 isolate) Relevancep24 is encoded by the HIV gag gene and is an important structural component of the capsid core of the HIV particle Cross reactivityNot tested SpecificityEpitope not mapped Tested applicationsWestern blots for clade B isolates of HIV; immunofluorescence assays; immunoprecipitation assays Raised in Mouse&n...

Human Thyrotrophin Receptor Monoclonal Antibody - Clone 4C1

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:54:24 GMT

Research tool forWestern blottingImmunoprecipitationFlow cytometryImmunohistochemistry (frozen section) Inhibits binding of TSH to TSH receptor by 70-80%. The human thyrotrophin receptor (TSHR) is found primarily in epithelial cells of thyroid follicles where it induces (by cAMP) the phospholipase C and protein kinase A signal transduction pathways. TSHR is the major pathogenic autoantigen in human autoimmune thyroid Graves’ disease. Product TypeHybridoma for monoclonal antibodyImmunogenBacterially expressed extracellular domain (residues 1-415 of humanTSHR fused to ...

HIV-1 integrase (IN) monoclonal antibody - Clone IN-3

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:53:27 GMT

Reagent nameHIV-1 integrase (IN) monoclonal antibody (#IN-3) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 integrase protein (clade B, HXB-3 isolate) RelevanceHIV-1 expresses the integrase (IN) protein as part of the polymerase gene. It is the viral enzyme that catalyses the insertion of viral DNA into the chromosomal DNA of an infected cells. Integrase is essential for viral growth and is a therapeutic target. Cross-reactivityNo cross-reactivity with no...

Human Thyrotrophin Receptor Monoclonal Antibody - Clone 3B12

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:53:17 GMT

Research tool for:Immunoblotting Immunoprecipitation Flow cytometry Immunohistochemistry (frozen section) Product TypeHybridoma for monoclonal antibodyImmunogenBacterially expressed extracellular domain (residues 1-415) of human TSHR fused to GSTCross ReactivityCross-reacts with human and theoretically with rat, pig, sheep, mouse, dog, catSpecificityDetects residues 336-345 of human TSHRRaised in MouseIsotypeIgG1 ReferencesShepherd PS, Da Costa CR, Cridland JC, Gilmore KS, Johnstone AP. 1999. Monoclonal antibodies that recognize the native human thyrotrophin receptor.&nbs...

HIV-1 p17 Gag monoclonal antibody - Clone 17-3

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:53:13 GMT

Reagent NameHIV-1 p17 Gag monoclonal antibody (#17-3) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 p17 Gag protein (clade B, HXB-3 isolate) Relevancep17 is encoded by the HIV gag gene and is an important structural component of the HIV particle Cross reactivityNot tested SpecificityEpitope not mapped Tested applicationsWestern blots for clade B isolates of HIV; immunoprecipitation assays; immunofluorescence/FACS assays Raised in Mouse ClonalityM...

HIV-1 Rev monoclonal antibody - Clone Rev-6

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:52:58 GMT

Reagent NameHIV-1 Rev monoclonal antibody (#Rev-6) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 Rev protein (clade B, HXB-3 isolate) RelevanceHIV-1 expresses the trans-activator protein Rev, which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. Cross-reactivityNo cross-reactivity with non-HIV-1 Rev proteins has been observed&nbs...

HIV-1 Vif Monoclonal Antibody - Clone 564

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:52:50 GMT

Reagent NameHIV-1 Vif monoclonal antibody (#564) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 Vif protein (clade B, HXB-3 isolate) RelevanceHIV-1 expresses the viral infectivity factor (vif) which induces productive viral replication by inactivating the host cell anti-HIV factor APOBEC3G Cross-reactivityNo cross-reactivity with non-HIV-1 Vif proteins has been observed SpecificityEpitope not yet mapped Tested applicationsImmunoprecipitation assays (not use...

Flow Cytometry-based Non-Transferrin-Bound Iron Assay

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:52:33 GMT

Fe-Flobead: A Sensitive High Throughput Test for Iron Overload An iron-sensitive, fluorescent, bead-based probe has been developed and incorporated into an assay for the accurate measurement of non-transferrin-bound iron (NTBI). The measurement of NTBI provides a valuable means for the early detection and follow-up of diseases associated with iron overload such as thalassaemia, sickle cell anaemia, myelodysplastic syndrome and haemochromatosis. Introduction NTBI refers to all forms of iron that are found in the plasma and that bind to ligands other than transferrin. NTBI is thought t...

Mouse anti-human Talin antibody - Clone TA205

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:52:26 GMT

Reagent NameMouse anti-human Talin antibody ImmunogenHuman platelet talin. RelevanceTalin is a cytoskeletal protein that is found concentrated in focal adhesions. It is capable of linking integrins to the actin cytoskeleton either directly or indirectly by interacting with vinculin and alpha-actinin Cross reactivityTA205 immunoblots a 225 kDa protein in human platelet lysates SpecificityEpitope resides in region 139-433 in talin. Tested ApplicationsImmunohistochemistry, Western blotting and immunoprecipitation. Raised inMouse ClonalityMonoclonal IsotypeI...

Mouse anti-human Glycoprotein 1b alpha (CD42b) antibody - Clone PM6/40.

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:52:06 GMT

Reagent NameMouse anti-human Glycoprotein 1b alpha antibody ImmunogenPlatelet membranes RelevanceGlycoprotein 1b (GP1b) is heavily expressed in platelets and megakaryocytes. It serves as a receptor for von Willebrand Factor and thrombin. Cross reactivityRecognises human GP1b, other species GP1b to be tested. SpecificityEpitope not mapped Tested ApplicationsFlow cytometry, immunoflourescence, immunohistochemistry (frozen sections) Raised inMouse ClonalityMonoclonal IsotypeIgG1 ReferencesThorsen LI et al 1998 Br. J. Haematol. 68, 67-74

Human Thyroglobulin Monoclonal Antibody - Clone 1D4

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:52:00 GMT

Research tool forELISARadioimmunoassayIn vivo imaging Thyroglobulin is a large iodinated glycoprotein which is produced by the thyroid gland, functioning as a prohormone in the synthesis of thyroxine (T4) and triiodothyronine (T3). Levels of serum thyroglobulin are elevated in a variety of thyroid diseases, such as Grave’s disease (goiter), subacute thyroiditis and differentiated thyroid carcinoma. Product TypeHybridoma for monoclonal antibodyImmunogenPurified human thyroglobulinCross ReactivityCross-reacts with baboon. Does not cross react with sheep, pig, bovine, ...

Targeting acute myeloid leukaemia with Poly-ADP-ribose polymerase (PARP) inhibitors.

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:51:29 GMT

SummaryAcute myeloid leukemia (AML) is one of the most common cancers, accounting for about 30,000 new cases each year in the US and EU. Despite recent advances in leukemia research, AML patients are still receiving the same treatment utilised over past few decades, and have a relatively poor prognosis, with 5 year overall survival rate of only about 25%. There is, an urgent need to design better therapeutic strategies to AML patients who are resistant to current therapies. Recent research has shown that synthetic-lethality using poly-(ADP-ribose)-polymerase (PARP) inhibitors...

SuperBUGS: Rabbit anti-MAP1B phospho (T1265)

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:51:26 GMT

Affinity purified anti-sera to the glycogen synthase kinase (GSK3b) mediated nonprimed phosphorylation site of Microtubule Associated Protein 1B (MAP1B). MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. King’s DeveloperProfessor Phillip R. Gordon-WeeksReagent nameSuperBUGS: Rabbit anti-MAP1B phospho (T1265)Immunogen (peptide sequence + location; recombinant protein; native protein from which species)CSPIEKTPLGER, in which threonine was phosphorylated, were synthesised and conjugated to k...

Mouse anti-human Glycoprotein 1b alpha (CD42b) antibody - Clone MM2/174.

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:51:19 GMT

Reagent NameMouse anti-human Glycoprotein 1b alpha (GP1b alpha subunit) antibody ImmunogenPlatelet membranes RelevanceGlycoprotein 1b is heavily expressed in platelets and megakaryocytes. It serves as a receptor for von Willebrandt Factor and thrombin. Cross reactivityRecognises human GP1b, other species to be tested. SpecificityEpitope not mapped. Tested ApplicationsWestern blotting, flow cytometry immunohistochemistry, immunofluorescence. Raised inMouse ClonalityMonoclonal IsotypeIgG1 ReferencesThorsen LI et al (1998) Br. J. Haematol. 68, 67-74

Human PMP22 Monoclonal Antibody

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:50:38 GMT

The First Human MAb developed specific for PMP22. Research tool for:Western blottingImmunohistochemistry – paraffin sectionsELISA Peripheral Myelin Protein 22 (PMP22) is a 160 amino acid glycoprotein which is a member of the claudin family and is thought to play an important role in the structure of peripheral nerve myelin. Irregularities in its gene results in hereditary demyelinating neuropathies. PMP22 protein is present in the compact myelin of adult human peripheral nerve and its distribution mimics that of Myelin Protein zero (P0). Product TypeHybridoma f...

Anti-CSPG4 antibody for the treatment of melanoma

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:50:35 GMT

SummaryA chimeric IgE class anti-CSPG4 antibody has been developed which has demonstrated positive results in vitro and in vivo. The antibody is designed to target immune responses directly against melanoma tumour cells, circumventing the broad immune activatory properties of currently approved immunotherapies. Figure: CSPG4 IgE restricts growth of an A375 human melanoma xenograft grown subcutaneously compared to CSPG4 IgG1 (**p<0.01) and non-specific antibody isotype controls when administered intravenously in a humanised mouse model with splenic engraftment of human immune effector c...

HIV-1 p17 Gag monoclonal antibody - Clone 17-2

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:50:08 GMT

Reagent NameHIV-1 p17 Gag monoclonal antibody (#17-2) DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 p17 Gag protein (clade B, HXB-3 isolate) Relevancep17 is encoded by the HIV gag gene and is an important structural component of the HIV particle Cross reactivityNot tested SpecificityEpitope not mapped Tested applicationsWestern blots for clade B isolates of HIV Raised in Mouse ClonalityMonoclonal IsotypeIgG1, k ReferencesFouchier RA, Me...

Superior Scalable Spatial Sound

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:49:55 GMT

“I would like to congratulate you on the apparent naturalness, at least from a first hearing on modest loudspeakers. The source movement was easily detectable and fairly easy to locate.”Francis RumseyChair, Technical Council at Audio Engineering Society “It was great to see how the project has evolved and I'm really impressed by the result. By way of comparison we checked out an ambisonic system later in the day. Having had your demo in the morning the lack of location precision in the ambisonic system was very apparent.”James Hall, Jawbone Inc. Imagine ...

HIV-1 monoclonal antibodies

inteum.test@kcl.ac.uk — Thu, 08 May 2014 00:49:49 GMT

Reagent NameHIV-1 monoclonal antibodies Clones availableClone 17-1 for HIV-1 p17 Gag MAbClone 17-2 for HIV-1 p17 Gag MAbClone 17-3 for HIV-1 p17 Gag MAbClone 24-3 for HIV-1 p24 Gag MAbClone 24-4 for HIV-1 p24 Gag MAbClone IN-2 for HIV-1 integrase (IN) MAbClone IN-3 for HIV-1 integrase (IN) MAbClone 564 for HIV-1 Vif MAbClone 319 for HIV-1 Vif MAbClone Rev-6 for HIV-1 Rev MAbClone Rev-4 for HIV-1 Rev MAb DescriptionHybridoma expressing antibodies to human immunodeficiency virus type 1 ImmunogenBacterially expressed, hexahistidine amino-terminal tagged HIV-1 p17 Gag, p24 Gag...