Affinity purified anti-sera to the glycogen synthase kinase (GSK3b) mediated nonprimed phosphorylation site of Microtubule Associated Protein 1B (MAP1B). MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones.
King’s Developer
Professor Phillip R. Gordon-Weeks
Reagent name
SuperBUGS: Rabbit anti-MAP1B phospho (T1265)
Immunogen (peptide sequence + location; recombinant protein; native protein from which species)
CSPIEKTPLGER, in which threonine was phosphorylated, were synthesised and conjugated to keyhole limpet haemocyanin
Epitope
SPIEKTPLGER in mouse MAP1B
Positive control (e.g. tissue/cell type or cell line)
Embryonic neurons, rodent neonatal brain extract
Raised in
Rabbit
Amount available if polyclonal
(Affinity purified) 4 ml
Cross reactivity
Mammals, chick, snake, frog, dogfish
Specificity
Phospho-specific
Tested Applications with suggested dilutions in specified applications
Immunoblotting (1:3,000), Immunostaining (tissue sections 1:900); Immunofluorescence (cell culture 1:200)
References
- Trivedi et al., 2005, J. Cell Sci. 118, 993-1005
- Scales et al., 2009, J. Cell Sci. 122, 2424-2435
