APOBEC3F is a human protein with natural antiviral activity, especially against HIV. As such, it is currently the subject of intense scientific investigation, and may lead to the development of new therapeutics useful for treating HIV/AIDS.
Expression and virion incorporation of APOBEC3F proteins
Immunoblot analysis of APOBEC3F wild-type (WT) and mutant proteins was performed on both 293T cells transfected with APOBEC3F expression plasmids and concentrated virus produced from these cells. Equivalent amounts of protein were loaded, as judged by amounts of the cellular protein 14-3-3 (top panel) and the viral protein p24CA (fourth panel). Band intensities of incorporated APOBEC3F proteins were measured first as relative to the p24CA loading control, and then the numbers shown are expressed as a proportion of the strongest band present on the gel (set at 100).

Reagent Name
Polyclonal antibody specific for the human-APOBEC3F protein
Description
Polyclonal serum raised in rabbits using a synthetic peptide immunogen
Immunogen
Synthetic peptide with an introduced Cys at the N terminus to allow coupling (CGLKYNFLFLDSKLQEILE)
Cross reactivity
Not tested
Specificity
Epitopes not mapped, but serum was raised against a synthetic peptide
Tested applications
Immunoblotting (1:1000)
Raised in
Rabbit
Clonality
Polyclonal
Isotype
N/A
Purification
Affinity chromatography
References
- Holmes KH, Koning FA, Bishop KN and Malim MH. APOBEC3F can inhibit the accumulation of HIV-1 reverse transcription products in the absence of hypermutation. Comparisons with APOBEC3G. J. Biol. Chem. Vol 282, 4, 2587-2595 (2007).