HIV-1 Vif Monoclonal Antibody - Clone 319

Reagent Name

HIV-1 Vif monoclonal antibody (#319)

Description

Hybridoma expressing antibodies to human immunodeficiency virus type 1

Immunogen

Bacterially expressed, hexahistidine amino-terminal tagged HIV-1 Vif protein (clade B, HXB-3 isolate)

Relevance

HIV-1 expresses the viral infectivity factor (vif) which induces productive viral replication by inactivating the host cell anti-HIV factor APOBEC3G

Cross-reactivity

No cross-reactivity with non-HIV-1 Vif proteins has been observed

Specificity

Epitope not yet mapped

Tested applications

Western blots; immunofluorescence assays

Raised in

Mouse

Clonality

Monoclonal

Isotype

IgG_{1, k}

References

1. Simon JH, Southerling TE, Peterson JC, Meyer BE, Malim MH. Complementation of vif-defective human immunodeficiency virus type 1 by primate, but not nonprimate, lentivirus vif genes. Journal of Virology 69:4166-4172, 1995.
2. Simon JH, Fouchier RA, Southerling TE, Guerra CB, Grant CK, Malim MH. The Vif and Gag proteins of human immunodeficiency virus type 1 colocalize in infected human T cells. Journal of Virology 71:5259-5267, 1997.
3. Sheehy AM, Gaddis NC, Malim MH. The anti-retroviral enzyme APOBEC3G is degraded by the proteasome in response to HIV-1 Vif. Nature Medicine 9:1404-1407, 2003.