APOBEC3G is a human protein with natural antiviral activity, especially against HIV. As such, it is currently the subject of intense scientific investigation, and may lead to the development of new therapeutics useful for treating HIV/AIDS.
Indirect Immunofluorescence
HeLa cell monolayers were transfected with an APOBEC3G expression vector, fixed after 24 hr and hybridised with the anti-APOBEC3G serum (1:500 dilution). Bound antibody was detected using an Alexa Fluor 594-conjugated goat secondary antibody and imaged by confocal microscopy. APOBEC3G is seen (in green) distributed throughout the cytosol, and concentrated in small areas called P-bodies.
Reagent name
Polyclonal antibody specific for the human-APOBEC3G protein
Description
Polyclonal serum raised in rabbits using a synthetic peptide immunogen
Immunogen
C-terminal peptide derived from human APOBEC3G (NH2-CPFQPWDGLDEHSQDLSGRLRAILQNQEN)
Cross reactivity
Not tested
Specificity
Epitopes not mapped, but serum was raised against a synthetic peptide
Tested applications
Immunoblotting (1:1000), indirect immunofluorescence (1:500)
Raised in
Rabbit
Clonality
Polyclonal
Isotype
N/A
References
- Gallois-Montbrun, S., Kramer, B., Swanson, C.M., Byers, H., Lynham, S., Ward, M. and Malim, M.H. (2007). Antiviral protein APOBEC3G localizes to ribonucleoprotein complexes found in P bodies and stress granules. Journal of Virology 81, 2165-2178.