Human Thyrotrophin Receptor Monoclonal Antibody - Clone 4C1

Research tool for

• Western blotting
• Immunoprecipitation
• Flow cytometry
• Immunohistochemistry (frozen section)

Inhibits binding of TSH to TSH receptor by 70-80%.

The human thyrotrophin receptor (TSHR) is found primarily in epithelial cells of thyroid follicles where it induces (by cAMP) the phospholipase C and protein kinase A signal transduction pathways.  TSHR is the major pathogenic autoantigen in human autoimmune thyroid Graves’ disease.

Product Type

Hybridoma for monoclonal antibody

Immunogen

Bacterially expressed extracellular domain (residues 1-415 of humanTSHR fused to GST)

CrossReactivity

Cross-reacts with human and theoretically with rat, pig, sheep, mouse, dog, cat

Specificity

Detects residues 378 - 384 of human TSHR.

Recognises nativeprotein.

Raised in

Mouse

Isotype

IgG_2a

References

1. Shepherd PS, Da Costa CR, Cridland JC, Gilmore KS, Johnstone AP. 1999. Monoclonal antibodies that recognize thenative human thyrotrophin receptor. MolCell Endocrinol. 149: 197-206.
2. Johnstone, AP, Cridland JC, Da Costa CR, Nussy SS, Shepherd. 2003. A functional site on the human thyrotrophinreceptor – potential therapeutic target in Graves’ disease. Clin Endocrinol. 59:437-441.